CRISPR-CAS9 TECHNOLOGY AND ITS APPLICATIONS IN BIOMEDICINE NATIONAL UNIVERSITY OF UZBEKISTAN NAMED AFTER MIRZO ULUGBEK
Keywords:
CRISPR-Cas9; genome editing; gene therapy; biomedicine; molecular biology; cancer treatment; functional genomics; regenerative medicine; ethical issues; genetic engineering.Abstract
CRISPR-Cas9 has emerged as one of the most transformative tools in molecular
biology, enabling precise, efficient, and programmable genome editing across a wide
variety of organisms. Originating as an adaptive immune system in prokaryotes,
CRISPR-Cas9 has become indispensable in modern biomedical research due to its
simplicity, high specificity, and low cost compared with earlier genome-editing
platforms such as TALENs and zinc-finger nucleases. This paper provides an in-depth
analysis of CRISPR-Cas9 mechanisms, current applications in biomedicine,
advancements in therapeutic genome editing, and ethical considerations.
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