STRUCTURAL AND IMMUNOHISTOCHEMICAL FEATURES OF THE NORMAL BRAIN AND THEIR MODULATION BY EXTERNAL FACTORS.
Keywords:
Keywords: brain, normative morphology, immunohistochemistry, neuron, glia, blood–brain barrier, markers, diagnostics, neuroprotectionAbstract
Abstract
This article provides a scientific analysis of the normative morphological and
immunohistochemical features of the brain, as well as the factors that influence them.
The normal structure of brain tissue, the distinctive morphological characteristics of
neurons and glial cells, the importance of the blood–brain barrier, and their role in
overall body functioning are thoroughly discussed. The article also examines the
possibilities of assessing the physiological state of the brain using
immunohistochemical methods that identify markers, proteins, and neurotransmitter
systems. The influence of external and internal factors (genetic predisposition, toxic
effects, inflammatory processes, stress, and environmental conditions) on brain
morphology and immunohistochemical indicators is analyzed based on scientific
literature. The results of this research serve as an important theoretical and practical
foundation for the early diagnosis and prevention of neurological diseases, as well as
for the development of neuroprotective therapies.
References
References
1. Avtandilov, G. G. (1990). Medical morphometry: A guide to quantitative methods
in morphology. Moscow: Medicine.
2. Yanin, V. L., & Co-authors. (2015). Practical methods in histological tissue
dehydration and paraffin embedding. St. Petersburg: Nauka.
3. Paxinos, G., & Watson, C. (2014). The Rat Brain in Stereotaxic Coordinates (7th
ed.). Academic Press.
4. Fawcett, D. W. (1994). A Textbook of Histology (12th ed.). New York: Chapman
& Hall.
5. Bancroft, J. D., & Gamble, M. (2019). Theory and Practice of Histological
Techniques (8th ed.). Elsevier Health Sciences.
6. Haines, D. E. (2017). Neuroanatomy: An Atlas of Structures, Sections, and Systems
(10th ed.). Wolters Kluwer.
7. Hall, J. E., & Guyton, A. C. (2020). Guyton and Hall Textbook of Medical
Physiology (14th ed.). Elsevier.
8. Ashwal, S., & Ferriero, D. M. (2019). Pathophysiology of brain injury and hypoxia
in experimental models. Journal of Cerebral Blood Flow & Metabolism, 39(3),
435–450.
9. Lo, E. H., Dalkara, T., & Moskowitz, M. A. (2003). Mechanisms, challenges, and
opportunities in stroke. Nature Reviews Neuroscience, 4(5), 399–415.
10. Dikmen, S. S., Corrigan, J. D., Levin, H. S., Machamer, J., Stiers, W., & Weis, K.
(2009). Cognitive outcome following traumatic brain injury. Journal of Head
Trauma Rehabilitation, 24(6), 430–438.
11. Eger, E. I., & Saidman, L. J. (2005). Pharmacology of inhaled anesthetics: Nitrous
oxide mechanisms revisited. Anesthesiology, 103(3), 660–671.
12. Jevtović-Todorović, V., Hartman, R. E., Izumi, Y., Benshoff, N. D., Dikranian, K.,
Zorumski, C. F., … Olney, J. W. (2003). Early exposure to common anesthetic
agents causes widespread neurodegeneration in developing rat brain. Journal of
Neuroscience, 23(3), 876–882.
13. Han, B. H., Xu, D., Choi, J., Han, Y., Xie, W., & De Caro, R. (2021). Role of
Caspase-3 activation in neural apoptosis after combined injury and anesthesia.
Neuroscience Letters, 753, 135879.
14. Sudhof, T. C. (2013). Neurotransmitter release: The synaptotagmin–synaptophysin
connection. Annual Review of Neuroscience, 36, 245–269.
15. Busija, D. W., & Heistad, D. D. (2018). The role of astrocytes and vascular
endothelium in cerebral ischemia. Brain Research Reviews, 126(2), 163–175.
16. Loane, D. J., & Kumar, A. (2016). Microglia in the TBI brain: The good, the bad,
and the dysregulated. Experimental Neurology, 275 Pt 3, 316–327.
17. Paxinos, G., Ashwell, K. W., & Törk, I. (1994). Atlas of the Developing Rat
Nervous System (2nd ed.). Academic Press.
18. Kerr, J. F. R., Wyllie, A. H., & Currie, A. R. (1972). Apoptosis: A basic biological
phenomenon with wide-ranging implications. British Journal of Cancer, 26(4),
239–257.
19. Squire, L. R., Berg, D., Bloom, F., du Lac, S., Ghosh, A., & Spitzer, N. C. (2012).
Fundamental Neuroscience (4th ed.). Academic Press.
20. Verkhratsky, A., & Butt, A. M. (2018). Glial Physiology and Pathophysiology (2nd
ed.). Wiley-Blackwell.
