TUPROQDAN AJRATIB OLINGAN TRICHODERMA HARZIANUMGA YAQIN ZAMBURUG‘ IZOLYATINING MORFOLOGIK VA MIKROMORFOLOGIK TAVSIFI
Keywords:
Kalit so‘zlar: Trichoderma harzianum, tuproq zamburug‘i, izolyat, sof kultura, mikologiya, biokontrol, Zomin tuprog‘iAbstract
ANNOTATSIYA
Mazkur tadqiqotda Jizzax viloyati Zomin tumani tuproqlaridan ajratib olingan
zamburug‘ izolyatining morfologik va mikromorfologik xususiyatlari kompleks tarzda
baholandi. Klassik mikologik usullar asosida tuproq namunalaridan 7 ta zamburug‘
koloniyasi ajratib olinib, ular orasidan yuqori o‘sish tezligiga ega izolyat tanlab olindi
va sof kulturaga o‘tkazildi. Olingan natijalar shuni ko‘rsatdiki, izolyat 25±2 °C
sharoitda 72 soatda 68.5±1.5 mm diametrga yetib, o‘rtacha 0.95 mm/soat tezlikda
radial o‘sish xususiyatini namoyon etdi. Koloniya dastlab oq rangli mitseliy hosil qilib,
keyinchalik markaziy qismida yashil sporulyatsiya zonasi shakllantirdi. Mikroskopik
tahlillar natijasida septali va kuchli tarmoqlangan gifalar (3.2±0.4 mkm), murakkab
shoxlangan konidioforlar hamda dumaloq/subgloboz konidiyalar (2.8–3.5 mkm)
mavjudligi aniqlandi. Ushbu makro- va mikromorfologik belgilar Trichoderma jinsiga,
xususan Trichoderma harzianum turiga xos diagnostik xususiyatlarga mos keladi.
Muhokama natijalari izolyatning yuqori o‘sish tezligi va sporulyatsiya faolligi uning
ekologik moslashuvchanligi hamda potensial biokontrol xususiyatlarini tasdiqlashini
ko‘rsatdi. Shunday qilib, ajratib olingan izolyat mahalliy agroekotizimlar uchun
istiqbolli biologik himoya agenti sifatida baholanadi, biroq tur darajasida aniq
identifikatsiya qilish uchun ITS-rDNK va tef1 markerlari asosida molekulyar-genetik
tadqiqotlar zarur.
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